| Title |
Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis
|
|---|---|
| Published in |
BMC Genomics, January 2011
|
| DOI | 10.1186/1471-2164-12-14 |
| Pubmed ID | |
| Authors |
Brady K Atwood, Jacqueline Lopez, James Wager-Miller, Ken Mackie, Alex Straiker |
| Abstract |
G protein coupled receptors (GPCRs) are one of the most widely studied gene superfamilies. Thousands of GPCR research studies have utilized heterologous expression systems such as human embryonic kidney cells (HEK293). Though often treated as 'blank slates', these cell lines nevertheless endogenously express GPCRs and related signaling proteins. The outcome of a given GPCR study can be profoundly influenced by this largely unknown complement of receptors and/or signaling proteins. Little easily accessible information exists that describes the expression profiles of the GPCRs in cell lines. What is accessible is often limited in scope - of the hundreds of GPCRs and related proteins, one is unlikely to find information on expression of more than a dozen proteins in a given cell line. Microarray technology has allowed rapid analysis of mRNA levels of thousands of candidate genes, but though often publicly available, the results can be difficult to efficiently access or even to interpret. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 617 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | <1% |
| Denmark | 3 | <1% |
| United Kingdom | 2 | <1% |
| Canada | 2 | <1% |
| Netherlands | 1 | <1% |
| Australia | 1 | <1% |
| Ukraine | 1 | <1% |
| Belgium | 1 | <1% |
| Portugal | 1 | <1% |
| Other | 4 | <1% |
| Unknown | 597 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 153 | 25% |
| Researcher | 101 | 16% |
| Student > Master | 76 | 12% |
| Student > Bachelor | 65 | 11% |
| Student > Doctoral Student | 31 | 5% |
| Other | 82 | 13% |
| Unknown | 109 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 162 | 26% |
| Biochemistry, Genetics and Molecular Biology | 118 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 67 | 11% |
| Neuroscience | 47 | 8% |
| Chemistry | 38 | 6% |
| Other | 58 | 9% |
| Unknown | 127 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2023.
All research outputs
#15,958,140
of 24,287,697 outputs
Outputs from BMC Genomics
#6,417
of 10,940 outputs
Outputs of similar age
#146,665
of 188,203 outputs
Outputs of similar age from BMC Genomics
#65
of 90 outputs
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