RT @BMATscientists: I’m constantly reviewing papers where Gapdh is used as a housekeeping gene, including in bone marrow & adipose tissues.…
RT @BMATscientists: I’m constantly reviewing papers where Gapdh is used as a housekeeping gene, including in bone marrow & adipose tissues.…
RT @BMATscientists: I’m constantly reviewing papers where Gapdh is used as a housekeeping gene, including in bone marrow & adipose tissues.…
I’m constantly reviewing papers where Gapdh is used as a housekeeping gene, including in bone marrow & adipose tissues. It’s TERRIBLE for these because it increases in adipogenesis. Please use other genes (& several of them, eg https://t.co/9K7ULv7
@UACachexia @davidjglassMD GeNorm could help for PCR? https://t.co/YFPlkVjKIM
RT @naomiattar: @drchristhorpe i started working there 10 years later and crazy how much the field had moved on - the TOCs in 2010 were ver…
@drchristhorpe i started working there 10 years later and crazy how much the field had moved on - the TOCs in 2010 were very different in flavour, to put it mildly!! that said, this 2002 gem from @jvdesomp remains one of GB's most accessed articles, even
@Ivalaine I run 13 reference genes and then Normfinder to find the 3 most stable, and calculate geometric mean for normalisation. https://t.co/7zboaQ9RKf
An excellent classic on qRT-PCR data analysis using multiple housekeeping genes is today's pick for #APaperADay. This @GenomeBiology paper has massive 20k plus citations. It presents a data normalization strategy for accurate RT-PCR expression profiling. h
RNA biology paper in today's #APaperADay challenge discusses the role of circRNAs in EMT of pre-eclampsia. They report that circTNRC18/miR-762/Grhl2 axis serves a key role in trophoblast cell migration and EMT and correlates to PE progression. https://t.co
In total, 387,000 accesses according to @GenomeBiology. Multigene #qPCR normalization alive and kicking. https://t.co/nES2uffI7N
@macauleylab A single reference gene is not acceptable, see MIQE guidelines for qPCR and https://t.co/nES2uffI7N
@susaopina Así es. La mejor forma de abordar honestamente este problema es,creo,metiendo varios genes de referencia en la ecuación. Son ya ~ 20 años desde que Vandesompele et al. lo dejan formalmente escrito. Para mi es un misterio cómo no ha calado aún
@TYMichaelsen Indeed, stable reference genes are proportionally expressed. https://t.co/nES2uffI7N
RT @jvdesomp: Correct! Three times rejected at editorial level. Thank you @StephenABustin for suggesting @GenomeBiology back in 2002! 13,83…
RT @jvdesomp: Correct! Three times rejected at editorial level. Thank you @StephenABustin for suggesting @GenomeBiology back in 2002! 13,83…
RT @jvdesomp: Correct! Three times rejected at editorial level. Thank you @StephenABustin for suggesting @GenomeBiology back in 2002! 13,83…
Correct! Three times rejected at editorial level. Thank you @StephenABustin for suggesting @GenomeBiology back in 2002! 13,832 citations according to GoogleScholar.
@DrAnneCarpenter @wolfgangkhuber @daniela_witten I believe geNorm from @jvdesomp had been sent elsewhere before it came to us - 10,100 citations so far. https://t.co/Ks776HUSlx
@tsano321 この考え方を実装するためのアルゴリズムはいくつかありますが、たとえばこの論文など。https://t.co/itkPC4arFM
@Point_Mutation single gene error >> systematic error https://t.co/3jr526nzYM https://t.co/tZToJY8JvB
@mikelove @notSoJunkDNA @BioMickWatson perhaps consider qPCR norm eg @jvdesomp 2002…use genes with minimal variation https://t.co/Y6WDdeT1sI
A nice paper on #normalization of #RT-#PCR #data http://t.co/QQ3aK9yybB #science #neuroscience #qPCR @BioRadLifeSci @LifeTech
A nice paper on #normalization of #RT-#PCR #data http://t.co/QQ3aK9yybB #science #neuroscience #qPCR @BioRadLifeSci @LifeTech
A nice paper on #normalization of #RT-#PCR #data http://t.co/QQ3aK9yybB #science #neuroscience #qPCR @BioRadLifeSci @LifeTech
@pookmainz The Vandesompele paper may be useful starting from the “Normalization factor calculation” heading http://t.co/O3QY9P0skE