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Genome and methylome of the oleaginous diatom Cyclotella cryptica reveal genetic flexibility toward a high lipid phenotype

Overview of attention for article published in Biotechnology for Biofuels, November 2016
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

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9 tweeters

Citations

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25 Dimensions

Readers on

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62 Mendeley
Title
Genome and methylome of the oleaginous diatom Cyclotella cryptica reveal genetic flexibility toward a high lipid phenotype
Published in
Biotechnology for Biofuels, November 2016
DOI 10.1186/s13068-016-0670-3
Pubmed ID
Authors

Jesse C. Traller, Shawn J. Cokus, David A. Lopez, Olga Gaidarenko, Sarah R. Smith, John P. McCrow, Sean D. Gallaher, Sheila Podell, Michael Thompson, Orna Cook, Marco Morselli, Artur Jaroszewicz, Eric E. Allen, Andrew E. Allen, Sabeeha S. Merchant, Matteo Pellegrini, Mark Hildebrand

Abstract

Improvement in the performance of eukaryotic microalgae for biofuel and bioproduct production is largely dependent on characterization of metabolic mechanisms within the cell. The marine diatom Cyclotella cryptica, which was originally identified in the Aquatic Species Program, is a promising strain of microalgae for large-scale production of biofuel and bioproducts, such as omega-3 fatty acids. We sequenced the nuclear genome and methylome of this oleaginous diatom to identify the genetic traits that enable substantial accumulation of triacylglycerol. The genome is comprised of highly methylated repetitive sequence, which does not significantly change under silicon starved lipid induction, and data further suggests the primary role of DNA methylation is to suppress DNA transposition. Annotation of pivotal glycolytic, lipid metabolism, and carbohydrate degradation processes reveal an expanded enzyme repertoire in C. cryptica that would allow for an increased metabolic capacity toward triacylglycerol production. Identification of previously unidentified genes, including those involved in carbon transport and chitin metabolism, provide potential targets for genetic manipulation of carbon flux to further increase its lipid phenotype. New genetic tools were developed, bringing this organism on a par with other microalgae in terms of genetic manipulation and characterization approaches. Functional annotation and detailed cross-species comparison of key carbon rich processes in C. cryptica highlights the importance of enzymatic subcellular compartmentation for regulation of carbon flux, which is often overlooked in photosynthetic microeukaryotes. The availability of the genome sequence, as well as advanced genetic manipulation tools enable further development of this organism for deployment in large-scale production systems.

Twitter Demographics

The data shown below were collected from the profiles of 9 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 2%
Unknown 61 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 23%
Student > Ph. D. Student 13 21%
Student > Master 13 21%
Unspecified 7 11%
Student > Bachelor 4 6%
Other 11 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 27 44%
Biochemistry, Genetics and Molecular Biology 13 21%
Unspecified 10 16%
Immunology and Microbiology 3 5%
Chemistry 2 3%
Other 7 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 December 2016.
All research outputs
#1,236,362
of 8,713,305 outputs
Outputs from Biotechnology for Biofuels
#99
of 713 outputs
Outputs of similar age
#57,364
of 299,492 outputs
Outputs of similar age from Biotechnology for Biofuels
#5
of 50 outputs
Altmetric has tracked 8,713,305 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 713 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,492 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 50 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.