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Casein kinase 1α has a non-redundant and dominant role within the CK1 family in melanoma progression

Overview of attention for article published in BMC Cancer, August 2016
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Title
Casein kinase 1α has a non-redundant and dominant role within the CK1 family in melanoma progression
Published in
BMC Cancer, August 2016
DOI 10.1186/s12885-016-2643-0
Pubmed ID
Authors

Tobias Sinnberg, Jun Wang, Birgit Sauer, Birgit Schittek

Abstract

We previously identified CK1α as a novel tumor suppressor in melanoma and reported that the loss of CK1α leads to increased proliferation and invasive growth of melanoma cells by strong activation of the Wnt/β-catenin signaling pathway. In this study we analyzed expression and the functional effects of the dominantly expressed CK1- isoforms α, δ and ε in melanoma cells by quantitative real-time PCR, western blot and immunohistochemistry. We down-regulated CK1 kinase activity with isoform specific siRNAs and small molecule inhibitors. Vice versa we overexpressed the CK1 isoforms α, δ and ε using viral vectors and tested the biological effects on melanoma cell proliferation, migration and invasion. We show that protein expression of all three CK1-isoforms is downregulated in metastatic melanoma cells compared to benign melanocytic cells. Furthermore, the CK1δ and ε isoforms are able to negatively regulate expression of each other, whereas CK1α expression is independently regulated in melanoma cells. Inhibition of the expression and activity of CK1δ or CK1ε by specific inhibitors or siRNAs had no significant effect on the growth and survival of metastatic melanoma cells. Moreover, the over-expression of CK1δ or CK1ε in melanoma cells failed to induce cell death and cell cycle arrest although p53 signaling was activated. This is in contrast to the effects of CK1α where up-regulated expression induces cell death and apoptosis in metastatic melanoma cells. These data indicate that CK1α has a dominant and non-redundant function in melanoma cells and that the CK1δ and ε isoforms are not substantially involved in melanoma progression.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 32%
Student > Master 3 16%
Student > Bachelor 3 16%
Student > Ph. D. Student 3 16%
Student > Doctoral Student 1 5%
Other 3 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 37%
Agricultural and Biological Sciences 5 26%
Medicine and Dentistry 3 16%
Pharmacology, Toxicology and Pharmaceutical Science 2 11%
Neuroscience 1 5%
Other 1 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2017.
All research outputs
#6,866,789
of 9,011,296 outputs
Outputs from BMC Cancer
#2,381
of 3,712 outputs
Outputs of similar age
#220,686
of 308,308 outputs
Outputs of similar age from BMC Cancer
#60
of 83 outputs
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