Title |
Production of human blood group B antigen epitope conjugated protein in Escherichia coli and utilization of the adsorption blood group B antibody
|
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Published in |
Microbial Cell Factories, August 2016
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DOI | 10.1186/s12934-016-0538-z |
Pubmed ID | |
Authors |
Wenjing Shang, Yafei Zhai, Zhongrui Ma, Gongjin Yang, Yan Ding, Donglei Han, Jiang Li, Houcheng Zhang, Jun Liu, Peng George Wang, Xian-wei Liu, Min Chen |
Abstract |
In the process of ABO-incompatible (ABOi) organ transplantation, removal of anti-A and/or B antibodies from blood plasma is a promising method to overcome hyperacute rejection and allograft loss caused by the immune response between anti-A and/or B antibodies and the A and/or B antigens in the recipient. Although there are commercial columns to do this work, the application is still limited because of the high production cost. In this study, the PglB glycosylation pathway from Campylobacter jejuni was exploited to produce glycoprotein conjugated with Escherichia coli O86:B7 O-antigen, which bears the blood group B antigen epitope to absorb blood group B antibody in blood. The titers of blood group B antibody were reduced to a safe level without changing the clotting function of plasma after glycoprotein absorption of B antibodies in the plasma. We developed a feasible strategy for the specific adsorption/removal of blood group antibodies. This method will be useful in ABOi organ transplantation and universal blood transfusion. |
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