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An ELISA assay using a combination of recombinant proteins from multiple strains of Orientia tsutsugamushi offers an accurate diagnosis for scrub typhus

Overview of attention for article published in BMC Infectious Diseases, June 2017
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Title
An ELISA assay using a combination of recombinant proteins from multiple strains of Orientia tsutsugamushi offers an accurate diagnosis for scrub typhus
Published in
BMC Infectious Diseases, June 2017
DOI 10.1186/s12879-017-2512-8
Pubmed ID
Authors

Chien-Chung Chao, Zhiwen Zhang, Tatyana Belinskaya, Wilawan Thipmontree, Wiwit Tantibhedyangkul, Saowaluk Silpasakorn, Ekkarat Wongsawat, Yupin Suputtamongkol, Wei-Mei Ching

Abstract

Scrub typhus (ST) is a disease caused by an obligate intracellular bacterium, Orientia tsutsugamushi, an organism that requires a BSL3 laboratory for propagation. The disease is hallmarked by an eschar at the site of the chigger bite, followed by the development of fever, malaise, myalgia, anorexia, and papulomacular rash. Indirect immunofluorescent assay (IFA) is the gold standard for scrub typhus diagnosis, however, the subjectivity of the assay, the need for a specialized laboratory and instruments has limited the wide use of the test in resource limited areas. A recombinant-protein based enzyme linked immunosorbent assay (ELISA) using the most abundant and immunodominant protein for the detection of Orientia specific antibodies in serum has been developed. The performance of the assay was evaluated using prospectively collected acute sera from 248 randomly selected patients in Thailand. The ELISA assay was evaluated using two different cutoff values. The receiver operating characteristic (ROC) curve generated cutoff values gave slightly better consistency with diagnosis of ST than those cutoff values established by averaging ELISA optical density of known negatives at 99% confidence interval. Both cutoff values provided similar statistical parameters when compared with the diagnosis of ST, indicating the validity of both calculations to derive cutoff values. These results suggest that both IgG and IgM ELISA performed well to accurately diagnose scrub typhus cases in endemic areas using only acute serum samples. We have successfully developed an ELISA assay for the detection of Orientia-specific antibodies in serum that could provide effective screening of acute sera under clinical setup and it is also a useful assay to estimate seroprevalence in various endemic areas.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 30%
Student > Bachelor 2 20%
Professor 1 10%
Lecturer 1 10%
Student > Ph. D. Student 1 10%
Other 2 20%
Readers by discipline Count As %
Medicine and Dentistry 3 30%
Agricultural and Biological Sciences 3 30%
Immunology and Microbiology 2 20%
Unspecified 2 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2017.
All research outputs
#9,087,637
of 11,352,109 outputs
Outputs from BMC Infectious Diseases
#3,044
of 4,222 outputs
Outputs of similar age
#192,247
of 267,159 outputs
Outputs of similar age from BMC Infectious Diseases
#69
of 107 outputs
Altmetric has tracked 11,352,109 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,222 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
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