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Management of enzyme diversity in high-performance cellulolytic cocktails

Overview of attention for article published in Biotechnology for Biofuels, June 2017
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  • Above-average Attention Score compared to outputs of the same age (55th percentile)
  • Average Attention Score compared to outputs of the same age and source

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2 tweeters

Citations

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7 Dimensions

Readers on

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28 Mendeley
Title
Management of enzyme diversity in high-performance cellulolytic cocktails
Published in
Biotechnology for Biofuels, June 2017
DOI 10.1186/s13068-017-0845-6
Pubmed ID
Authors

Francisco Manuel Reyes-Sosa, Macarena López Morales, Ana Isabel Platero Gómez, Noelia Valbuena Crespo, Laura Sánchez Zamorano, Javier Rocha-Martín, Fernando P. Molina-Heredia, Bruno Díez García, Francisco Manuel Reyes-Sosa, Macarena López Morales, Ana Isabel Platero Gómez, Noelia Valbuena Crespo, Laura Sánchez Zamorano, Javier Rocha-Martín, Fernando P. Molina-Heredia, Bruno Díez García

Abstract

Modern biorefineries require enzymatic cocktails of improved efficiency to generate fermentable sugars from lignocellulosic biomass. Cellulolytic fungi, among other microorganisms, have demonstrated the highest potential in terms of enzymatic productivity, complexity and efficiency. On the other hand, under cellulolytic-inducing conditions, they often produce a considerable diversity of carbohydrate-active enzymes which allow them to adapt to changing environmental conditions. However, industrial conditions are fixed and adjusted to the optimum of the whole cocktail, resulting in underperformance of individual enzymes. One of these cellulolytic cocktails from Myceliophthora thermophila has been analyzed here by means of LC-MS/MS. Pure GH6 family members detected have been characterized, confirming previous studies, and added to whole cocktails to compare their contribution in the hydrolysis of industrial substrates. Finally, independent deletions of two GH6 family members, as an example of the enzymatic diversity management, led to the development of a strain producing a more efficient cellulolytic cocktail. These data indicate that the deletion of noncontributive cellulases (here EG VI) can increase the cellulolytic efficiency of the cocktail, validating the management of cellulase diversity as a strategy to obtain improved fungal cellulolytic cocktails.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Thailand 1 4%
Unknown 27 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 21%
Student > Ph. D. Student 5 18%
Unspecified 3 11%
Student > Bachelor 3 11%
Student > Master 2 7%
Other 9 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 32%
Agricultural and Biological Sciences 9 32%
Unspecified 6 21%
Engineering 2 7%
Materials Science 1 4%
Other 1 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 June 2017.
All research outputs
#6,131,999
of 11,400,239 outputs
Outputs from Biotechnology for Biofuels
#389
of 871 outputs
Outputs of similar age
#111,767
of 263,543 outputs
Outputs of similar age from Biotechnology for Biofuels
#17
of 34 outputs
Altmetric has tracked 11,400,239 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 871 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 53% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,543 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.