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Antibacterial activity and effects of Colla corii asini on Salmonella typhimurium invasion in vitro and in vivo

Overview of attention for article published in BMC Complementary and Alternative Medicine, December 2017
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Title
Antibacterial activity and effects of Colla corii asini on Salmonella typhimurium invasion in vitro and in vivo
Published in
BMC Complementary and Alternative Medicine, December 2017
DOI 10.1186/s12906-017-2020-9
Pubmed ID
Authors

Kwang-il Park, Mi-ra Lee, Tae-woo Oh, Kwang-Youn Kim, Jin-yeul Ma

Abstract

Salmonella enterica serovar Typhimurium is a foodborne pathogen that triggers inflammatory responses in the intestines of humans and livestock. Colla corii asini is a traditional medicine used to treat gynecologic and chronic diseases in Korea and China. However, the antibacterial activity of Colla corii asini has been unknown. In this study, we investigated the antibacterial activity and effects of Colla corii asini extract on Salmonella typhimurium invasion. To tested for antibacterial effects of Colla corii asini extracts, we confirmed the agar diffusion using Luria solid broth medium. Also, we determined the MIC (minimum inhibitory concentration) and the MBC (minimum bactericidal concentration) value of the Colla corii asini ethanol extract (CEE) by using two-fold serial dilution methods. We evaluated the expression of salmonella invasion proteins including SipA, SipB and SipC by using Western blot and qPCR at the concentration of CEE without inhibition of bacterial growth. In vitro and vivo, we determined the inhibitory effect of invasion of S. typhimurium on CEE by using gentamicin assay and S. typhimurium-infected mice. CEE significantly inhibited the growth of Salmonella typhimurium in an agar diffuse assay and had an MIC of 0.78 mg/ml and an MBC of 1.56 mg/ml. Additionally, CEE reduced Salmonella typhimurium cell invasion via the inhibition of Salmonella typhimurium invasion proteins, such as SipA, SipB and SipC. Furthermore, CEE significantly suppressed invasion in the small intestines (ilea) of mice injected with Salmonella typhimurium. These findings show that Colla corii asini exerts antibacterial activity and suppresses Salmonella typhimurium invasion in vitro and in vivo. Together, these findings demonstrate that Colla corii asini is a potentially useful therapeutic herbal medicine for treating salmonella-mediated diseases.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Student > Master 3 30%
Student > Bachelor 2 20%
Student > Doctoral Student 1 10%
Unspecified 1 10%
Other 0 0%
Readers by discipline Count As %
Unspecified 3 30%
Biochemistry, Genetics and Molecular Biology 2 20%
Agricultural and Biological Sciences 2 20%
Veterinary Science and Veterinary Medicine 1 10%
Economics, Econometrics and Finance 1 10%
Other 1 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2018.
All research outputs
#10,368,126
of 12,996,276 outputs
Outputs from BMC Complementary and Alternative Medicine
#1,811
of 2,618 outputs
Outputs of similar age
#275,675
of 383,405 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#234
of 403 outputs
Altmetric has tracked 12,996,276 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,618 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one is in the 17th percentile – i.e., 17% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 383,405 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 403 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.