| Title |
Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, April 2018
|
| DOI | 10.1186/s12906-018-2176-y |
| Pubmed ID | |
| Authors |
Raid Al Akeel, Ayesha Mateen, Khalid K. Alharbi, Abdullah A. Alyousef, Hazem M. Al-Mandeel, Rabbani Syed |
| Abstract |
Cucumis sativus L. (cucumber), from the family Cucurbitaceae, is a therapeutic plant with various pharmacological benefits, broadly utilized as a part of complementary medicine (e.g., Unani, Ayurveda, Siddha, and Traditional Chinese). In light of past research discoveries, this plant had been chosen to consider its potential antibacterial action. Extracts were purified by dialysis and ion exchange chromatography strategy and then assayed for antibacterial activity against four standard pathogenic bacterial strains known to cause foodborne infections and spoilage of food and herbal drugs. Antimicrobial peptides were extracted from seeds using a sodium phosphate citrate (pH 7.2) - CTAB cradle (pH 6.0). The highest protein concentration was seen with elute fractions 1 and 3 (370 mg/mL) compared with elute fractions 2 and 4 (340 mg/mL). Among the bacteria utilized, E. coli was clearly the most sensitive out of selected four strains. Our results suggest that Cucumis sativus L seeds extracts have significant potentials as new antimicrobial agents. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 54 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 9 | 17% |
| Researcher | 6 | 11% |
| Other | 4 | 7% |
| Student > Ph. D. Student | 4 | 7% |
| Student > Master | 4 | 7% |
| Other | 8 | 15% |
| Unknown | 19 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 13% |
| Chemistry | 5 | 9% |
| Biochemistry, Genetics and Molecular Biology | 5 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 7% |
| Medicine and Dentistry | 4 | 7% |
| Other | 9 | 17% |
| Unknown | 20 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 April 2018.
All research outputs
#20,480,611
of 23,041,514 outputs
Outputs from BMC Complementary Medicine and Therapies
#2,993
of 3,646 outputs
Outputs of similar age
#290,539
of 329,118 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#59
of 93 outputs
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So far Altmetric has tracked 3,646 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 93 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.