Title |
False negative results from using common PCR reagents
|
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Published in |
BMC Research Notes, October 2011
|
DOI | 10.1186/1756-0500-4-457 |
Pubmed ID | |
Authors |
Dean J Bacich, Kathryn M Sobek, Jessica L Cummings, Allison A Atwood, Denise S O'Keefe |
Abstract |
The sensitivity of the PCR reaction makes it ideal for use when identifying potentially novel viral infections in human disease. Unfortunately, this same sensitivity also leaves this popular technique open to potential contamination with previously amplified PCR products, or "carry-over" contamination. PCR product carry-over contamination can be prevented with uracil-DNA-glycosylase (UNG), and it is for this reason that it is commonly included in many commercial PCR master-mixes. While testing the sensitivity of PCR assays to detect murine DNA contamination in human tissue samples, we inadvertently discovered that the use of this common PCR reagent may lead to the production of false-negative PCR results. |
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Mendeley readers
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