↓ Skip to main content

False negative results from using common PCR reagents

Overview of attention for article published in BMC Research Notes, October 2011
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

Mentioned by

twitter
8 X users

Citations

dimensions_citation
34 Dimensions

Readers on

mendeley
124 Mendeley
Title
False negative results from using common PCR reagents
Published in
BMC Research Notes, October 2011
DOI 10.1186/1756-0500-4-457
Pubmed ID
Authors

Dean J Bacich, Kathryn M Sobek, Jessica L Cummings, Allison A Atwood, Denise S O'Keefe

Abstract

The sensitivity of the PCR reaction makes it ideal for use when identifying potentially novel viral infections in human disease. Unfortunately, this same sensitivity also leaves this popular technique open to potential contamination with previously amplified PCR products, or "carry-over" contamination. PCR product carry-over contamination can be prevented with uracil-DNA-glycosylase (UNG), and it is for this reason that it is commonly included in many commercial PCR master-mixes. While testing the sensitivity of PCR assays to detect murine DNA contamination in human tissue samples, we inadvertently discovered that the use of this common PCR reagent may lead to the production of false-negative PCR results.

X Demographics

X Demographics

The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 <1%
Germany 1 <1%
Singapore 1 <1%
Unknown 121 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 40 32%
Student > Ph. D. Student 13 10%
Researcher 12 10%
Student > Master 12 10%
Student > Doctoral Student 7 6%
Other 9 7%
Unknown 31 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 27 22%
Biochemistry, Genetics and Molecular Biology 25 20%
Immunology and Microbiology 9 7%
Medicine and Dentistry 6 5%
Veterinary Science and Veterinary Medicine 5 4%
Other 20 16%
Unknown 32 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 September 2020.
All research outputs
#4,292,611
of 23,314,015 outputs
Outputs from BMC Research Notes
#648
of 4,307 outputs
Outputs of similar age
#24,498
of 141,887 outputs
Outputs of similar age from BMC Research Notes
#8
of 56 outputs
Altmetric has tracked 23,314,015 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,307 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 141,887 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 56 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.