@UrnovFyodor 1/ Large deletions are the most prevalent for single cut. 2/ Much less with ssDNA donor compared to dsDNA but still there I am sure this paper would be your interest and reports the prevalence of ectopic insertion with lssDNA in mice http
In case you missed it: Application of long #single-stranded DNA donors in #genome editing: generation and validation of #mouse mutants https://t.co/t9JIqsmswd Genome editing techniques shift the challenge of #mutagenesis from generation to the validation
RT @GaetanBurgio: @UrnovFyodor Yep but ssDNA are not immune too of random integrations in the genome, although significantly less than dsDN…
@UrnovFyodor Yep but ssDNA are not immune too of random integrations in the genome, although significantly less than dsDNA https://t.co/Tdds6DAbzS
RT @BMCBiology: From Lydia Teboul and colleagues - Conditional alleles, and point #mutations generated by long single-stranded #DNA donors…
RT @BMCBiology: Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants https://t.co/h…
#CRISPR mutants need careful validation for incorrect and multiple insertions - a great example here! "Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants" https://t.co/JKXWGfHUSR
RT @BMCBiology: From Lydia Teboul and colleagues - Conditional alleles, and point #mutations generated by long single-stranded #DNA donors…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
RT @LluisMontoliu: Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in th…
Perhaps easi #CRISPR is not that easy. The powerful strategy developed by @guruccb & @Masato_Ohtsuka is challenged in the most recent work by Lydia Teboul and colleagues from @MRCHarwell Unexpected mutations and unexpected random integration of long ss
RT @MRCHarwell: Research published in @BMCBiology today, efficiency and reliability of long single-stranded DNA donors in #CRISPR/#Cas9 htt…
RT @MRCHarwell: Research published in @BMCBiology today, efficiency and reliability of long single-stranded DNA donors in #CRISPR/#Cas9 htt…
RT @MRCHarwell: Research published in @BMCBiology today, efficiency and reliability of long single-stranded DNA donors in #CRISPR/#Cas9 htt…
RT @BMCBiology: From Lydia Teboul and colleagues - Conditional alleles, and point #mutations generated by long single-stranded #DNA donors…
RT @MRCHarwell: Research published in @BMCBiology today, efficiency and reliability of long single-stranded DNA donors in #CRISPR/#Cas9 htt…
RT @BMCBiology: From Lydia Teboul and colleagues - Conditional alleles, and point #mutations generated by long single-stranded #DNA donors…
RT @BMCBiology: From Lydia Teboul and colleagues - Conditional alleles, and point #mutations generated by long single-stranded #DNA donors…
RT @MRCHarwell: Research published in @BMCBiology today, efficiency and reliability of long single-stranded DNA donors in #CRISPR/#Cas9 htt…
RT @BMCBiology: From Lydia Teboul and colleagues - Conditional alleles, and point #mutations generated by long single-stranded #DNA donors…
RT @BMCBiology: From Lydia Teboul and colleagues - Conditional alleles, and point #mutations generated by long single-stranded #DNA donors…
IoN UCL PubMed: Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants. https://t.co/wLR9IhCKP9
Research published in @BMCBiology today, efficiency and reliability of long single-stranded DNA donors in #CRISPR/#Cas9 https://t.co/qpE3AaJ6w4 #genetics @ICMNewcastle @UCLBrainScience https://t.co/WE0K8OZcda
RT @BMCBiology: From Lydia Teboul and colleagues - Conditional alleles, and point #mutations generated by long single-stranded #DNA donors…
RT @BMCBiology: Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants https://t.co/h…
Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants https://t.co/h59pPeUzpk