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Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo

Overview of attention for article published in BMC Complementary and Alternative Medicine, November 2015
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Title
Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo
Published in
BMC Complementary and Alternative Medicine, November 2015
DOI 10.1186/s12906-015-0926-7
Pubmed ID
Authors

Nawal Al-Henhena, Shaden A. M. Khalifa, Rozaida Poh Yuen Ying, Salmah Ismail, Riad Hamadi, Abdrabu N. Shawter, Azila Mohd Idris, Ainnul Azizan, Nahla Saeed Al-Wajeeh, Mahmood Ameen Abdulla, Hesham R. El-Seedi

Abstract

With cancer being one of the major causes of death around the world, studies are ongoing to find new chemotherapeutic leads. There are common mechanisms for colorectal cancer (CRC) formation. Several are connected with oxidative stress-induced cell apoptosis and others are related to imbalanced homeostasis or intake of drugs/toxins. Plants that have been used for decades in folk and traditional medicine have been accepted as one of the commonest sources of discovered natural agents of cancer chemotherapy and chemoprevention. The aim was to study the antioxidant and chemopreventive effects of Strobilanthes crispus on colorectal cancer formation. Five groups of rats were injected subcutaneously with AOM, 15 mg/kg body weight, each once weekly for 2 weeks. The cancer group was continued on 10 % Tween-20 feeding for 8 weeks. The standard drug group was continued on 35 mg/kg 5-fluorouracil intraperitoneal injection twice a week for 8 weeks, and the experimental groups were continued on 250 and 500 mg/kg S. crispus extract oral feeding for 8 weeks, respectively. The normal group was injected subcutaneously with normal saline once a week for 2 weeks, followed by oral administration of 10 % Tween-20 for 8 weeks. All the rats were sacrificed after 10 weeks. The colons were evaluated grossly and histopathologically for aberrant crypt foci (ACF). Gene expression was performed for Bax, Bcl2, Defa24, Slc24a3, and APC genes by real-time PCR. S. crispus and its fractions were evaluated for their chemopreventive effects against human colorectal adenocarcinoma cell line HT29 and cytotoxicity for normal human colon epithelial cell line CCD 841, and the active fraction was assessed for its components. We observed significant decrease in total colonic ACF formation, malonaldehyde (MDA) and lactate dehydrogenase (LDH), increase in superoxide dismutase (SOD), up-regulation of APC, Bax and Slc24a3, and down-regulation of Defa24 and Bcl-2 in rats treated with Strobilanthes crispus. Our results support the in vivo protection of S. crispus against CRC formation (azoxymethane-induced aberrant crypt foci) and suggest that the mechanism is highly specific to protect from oxidative insults and the following apoptotic cascade.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 28%
Student > Bachelor 5 20%
Unspecified 4 16%
Student > Master 3 12%
Student > Doctoral Student 2 8%
Other 4 16%
Readers by discipline Count As %
Unspecified 6 24%
Agricultural and Biological Sciences 4 16%
Medicine and Dentistry 4 16%
Pharmacology, Toxicology and Pharmaceutical Science 3 12%
Chemistry 2 8%
Other 6 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 November 2015.
All research outputs
#5,643,458
of 6,613,942 outputs
Outputs from BMC Complementary and Alternative Medicine
#1,491
of 1,747 outputs
Outputs of similar age
#198,818
of 251,103 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#57
of 81 outputs
Altmetric has tracked 6,613,942 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,747 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 251,103 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 81 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.