| Title |
Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, November 2015
|
| DOI | 10.1186/s12906-015-0926-7 |
| Pubmed ID | |
| Authors |
Nawal Al-Henhena, Shaden A. M. Khalifa, Rozaida Poh Yuen Ying, Salmah Ismail, Riad Hamadi, Abdrabu N. Shawter, Azila Mohd Idris, Ainnul Azizan, Nahla Saeed Al-Wajeeh, Mahmood Ameen Abdulla, Hesham R. El-Seedi |
| Abstract |
With cancer being one of the major causes of death around the world, studies are ongoing to find new chemotherapeutic leads. There are common mechanisms for colorectal cancer (CRC) formation. Several are connected with oxidative stress-induced cell apoptosis and others are related to imbalanced homeostasis or intake of drugs/toxins. Plants that have been used for decades in folk and traditional medicine have been accepted as one of the commonest sources of discovered natural agents of cancer chemotherapy and chemoprevention. The aim was to study the antioxidant and chemopreventive effects of Strobilanthes crispus on colorectal cancer formation. Five groups of rats were injected subcutaneously with AOM, 15 mg/kg body weight, each once weekly for 2 weeks. The cancer group was continued on 10 % Tween-20 feeding for 8 weeks. The standard drug group was continued on 35 mg/kg 5-fluorouracil intraperitoneal injection twice a week for 8 weeks, and the experimental groups were continued on 250 and 500 mg/kg S. crispus extract oral feeding for 8 weeks, respectively. The normal group was injected subcutaneously with normal saline once a week for 2 weeks, followed by oral administration of 10 % Tween-20 for 8 weeks. All the rats were sacrificed after 10 weeks. The colons were evaluated grossly and histopathologically for aberrant crypt foci (ACF). Gene expression was performed for Bax, Bcl2, Defa24, Slc24a3, and APC genes by real-time PCR. S. crispus and its fractions were evaluated for their chemopreventive effects against human colorectal adenocarcinoma cell line HT29 and cytotoxicity for normal human colon epithelial cell line CCD 841, and the active fraction was assessed for its components. We observed significant decrease in total colonic ACF formation, malonaldehyde (MDA) and lactate dehydrogenase (LDH), increase in superoxide dismutase (SOD), up-regulation of APC, Bax and Slc24a3, and down-regulation of Defa24 and Bcl-2 in rats treated with Strobilanthes crispus. Our results support the in vivo protection of S. crispus against CRC formation (azoxymethane-induced aberrant crypt foci) and suggest that the mechanism is highly specific to protect from oxidative insults and the following apoptotic cascade. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 66 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 13 | 20% |
| Student > Ph. D. Student | 11 | 17% |
| Student > Master | 6 | 9% |
| Student > Doctoral Student | 4 | 6% |
| Researcher | 3 | 5% |
| Other | 6 | 9% |
| Unknown | 23 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 7 | 11% |
| Agricultural and Biological Sciences | 6 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 8% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Immunology and Microbiology | 3 | 5% |
| Other | 12 | 18% |
| Unknown | 28 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 November 2015.
All research outputs
#18,431,664
of 22,834,308 outputs
Outputs from BMC Complementary Medicine and Therapies
#2,512
of 3,631 outputs
Outputs of similar age
#278,925
of 386,751 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#47
of 74 outputs
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