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Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology

Overview of attention for article published in Plant Methods, January 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • Good Attention Score compared to outputs of the same age and source (66th percentile)

Mentioned by

10 tweeters
1 patent
1 Wikipedia page


75 Dimensions

Readers on

381 Mendeley
Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology
Published in
Plant Methods, January 2010
DOI 10.1186/1746-4811-6-1
Pubmed ID

Jian-Feng Li, Li, Jen Sheen


Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed for a particular type of DNA extraction work, and the cost of purchasing these kits over a long run can be considerable. Furthermore, a simple method for the isolation of binary plasmid from Agrobacterium tumefaciens cells with satisfactory yield is lacking. Here we describe an easy protocol using homemade silicon dioxide matrix and seven simple solutions for DNA extraction from E. coli and A. tumefaciens cells, PCR and restriction digests, agarose gel slices, and plant tissues. Compared with the commercial kits, this protocol allows rapid DNA purification from diverse sources with comparable yield and purity at negligible cost. Following this protocol, we have demonstrated: (1) DNA fragments as small as a MYC-epitope tag coding sequence can be successfully recovered from an agarose gel slice; (2) Miniprep DNA from E. coli can be eluted with as little as 5 mul water, leading to high DNA concentrations (>1 mug/mul) for efficient biolistic bombardment of Arabidopsis seedlings, polyethylene glycol (PEG)-mediated Arabidopsis protoplast transfection and maize protoplast electroporation; (3) Binary plasmid DNA prepared from A. tumefaciens is suitable for verification by restriction analysis without the need for large scale propagation; (4) High-quality genomic DNA is readily isolated from several plant species including Arabidopsis, tobacco and maize. Thus, the silicon dioxide matrix-based DNA purification protocol offers an easy, efficient and economical way to extract DNA for various purposes in plant research.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 381 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 4 1%
United States 4 1%
Brazil 4 1%
France 2 <1%
United Kingdom 2 <1%
Argentina 2 <1%
Kenya 1 <1%
South Africa 1 <1%
Austria 1 <1%
Other 8 2%
Unknown 352 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 95 25%
Student > Ph. D. Student 83 22%
Student > Master 48 13%
Student > Bachelor 34 9%
Professor > Associate Professor 17 4%
Other 62 16%
Unknown 42 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 232 61%
Biochemistry, Genetics and Molecular Biology 53 14%
Engineering 11 3%
Chemistry 7 2%
Environmental Science 7 2%
Other 20 5%
Unknown 51 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2022.
All research outputs
of 23,511,526 outputs
Outputs from Plant Methods
of 1,120 outputs
Outputs of similar age
of 166,842 outputs
Outputs of similar age from Plant Methods
of 6 outputs
Altmetric has tracked 23,511,526 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,120 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 166,842 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.