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Validation of western blot for Histoplasma capsulatum antibody detection assay

Overview of attention for article published in BMC Infectious Diseases, February 2016
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1 tweeter
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Citations

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28 Mendeley
Title
Validation of western blot for Histoplasma capsulatum antibody detection assay
Published in
BMC Infectious Diseases, February 2016
DOI 10.1186/s12879-016-1427-0
Pubmed ID
Authors

Marcos de Abreu Almeida, Cláudia Vera Pizzini, Lisandra Serra Damasceno, Mauro de Medeiros Muniz, Rodrigo Almeida-Paes, Regina Helena Saramago Peralta, José Mauro Peralta, Raquel de Vasconcelos Carvalhaes Oliveira, Alexandre Gomes Vizzoni, Carla Lourenço Tavares de Andrade, Rosely Maria Zancopé-Oliveira

Abstract

Histoplasmosis is worldwide systemic mycoses caused by the dimorphic fungus Histoplasma capsulatum. The isolation and identification of H. capsulatum in culture is the reference test for histoplasmosis diagnosis confirmation. However, in the absence of it, serology has been used as a presumptive diagnosis through antibody and antigen detection. The purpose of the present study was to validate an immunoassay method (western blot) for antibodies detection in the diagnosis of histoplasmosis. To validate the western blot (WB) a study was conducted using 118 serum samples from patients with histoplasmosis and 118 serum controls collected from January 2000 to December 2013 in residents of the Rio de Janeiro State, Brazil. Diagnostic validation parameters were calculated based on the categorization of results obtained in a 2 × 2 table and subjected to statistical analysis. In addition, the viability of deglycosylated histoplasmin antigen (ptHMIN) onto nitrocellulose membranes previously sensitized was evaluated during the same period. The WB test showed sensitivity of 94.9 %, specificity of 94.1 %, positive predictive value of 94.1 %, negative predictive value of 94.9 %, accuracy of 94.5 %, and almost perfect precision. Besides, the strips have proved to be viable for using at least 5 years after ptHMIN antigen sensitization. Western blot test using ptHMIN provides sensitive, specific, and faster results. Therefore, could be considered a useful tool in the diagnosis of histoplasmosis being used by public health system, even in situations where laboratory facilities are relatively limited.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 4%
Unknown 27 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 32%
Unspecified 5 18%
Student > Master 4 14%
Student > Postgraduate 2 7%
Lecturer 2 7%
Other 6 21%
Readers by discipline Count As %
Unspecified 7 25%
Immunology and Microbiology 4 14%
Biochemistry, Genetics and Molecular Biology 4 14%
Medicine and Dentistry 3 11%
Agricultural and Biological Sciences 3 11%
Other 7 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 June 2017.
All research outputs
#9,711,689
of 12,657,345 outputs
Outputs from BMC Infectious Diseases
#2,999
of 4,710 outputs
Outputs of similar age
#170,399
of 266,664 outputs
Outputs of similar age from BMC Infectious Diseases
#4
of 4 outputs
Altmetric has tracked 12,657,345 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,710 research outputs from this source. They receive a mean Attention Score of 4.8. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
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