Title |
MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
|
---|---|
Published in |
BMC Molecular and Cell Biology, September 2010
|
DOI | 10.1186/1471-2199-11-74 |
Pubmed ID | |
Authors |
Stephen A Bustin, Jean-François Beaulieu, Jim Huggett, Rolf Jaggi, Frederick SB Kibenge, Pål A Olsvik, Louis C Penning, Stefan Toegel |
Abstract |
The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols is leading to the publication of papers that may not be technically appropriate. We take the view that this problem requires the delineation of a more transparent and comprehensive reporting policy from scientific journals. This editorial aims to provide practical guidance for the incorporation of absolute minimum standards encompassing the key assay parameters for accurate design, documentation and reporting of qPCR experiments (MIQE précis) and guidance on the publication of pure 'reference gene' articles. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 7 | <1% |
Mexico | 6 | <1% |
Brazil | 6 | <1% |
Germany | 5 | <1% |
France | 4 | <1% |
United Kingdom | 4 | <1% |
Spain | 3 | <1% |
South Africa | 3 | <1% |
Chile | 2 | <1% |
Other | 18 | 2% |
Unknown | 838 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 226 | 25% |
Researcher | 182 | 20% |
Student > Master | 123 | 14% |
Student > Bachelor | 58 | 6% |
Student > Doctoral Student | 49 | 5% |
Other | 166 | 19% |
Unknown | 92 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 477 | 53% |
Biochemistry, Genetics and Molecular Biology | 126 | 14% |
Medicine and Dentistry | 58 | 6% |
Environmental Science | 20 | 2% |
Engineering | 17 | 2% |
Other | 80 | 9% |
Unknown | 118 | 13% |