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Multiple linear epitopes (B-cell, CTL and Th) of JEV expressed in recombinant MVA as multiple epitope vaccine induces a protective immune response

Overview of attention for article published in Virology Journal, January 2012
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Title
Multiple linear epitopes (B-cell, CTL and Th) of JEV expressed in recombinant MVA as multiple epitope vaccine induces a protective immune response
Published in
Virology Journal, January 2012
DOI 10.1186/1743-422x-9-204
Pubmed ID
Authors

Fengjuan Wang, Xiuli Feng, Qisheng Zheng, Hongyan Hou, Ruibing Cao, Bin Zhou, Qingtao Liu, Xiaodong Liu, Ran Pang, Jin Zhao, Wenlei Deng, Puyan Chen

Abstract

Epitope-based vaccination might play an important role in the protective immunity against Japanese encephalitis virus (JEV) infection. The purpose of the study is to evaluate the immune characteristics of recombinant MVA carrying multi-epitope gene of JEV (rMVA-mep). The synthetic gene containing critical epitopes (B-cell, CTL and Th) of JEV was cloned into the eukaryotic expression vector pGEM-K1L, and the rMVA-mep was prepared. BALB/c mice were immunized with different dosages of purified rMVA-mep and the immune responses were determined in the form of protective response against JEV, antibodies titers (IgG1 and IgG2a), spleen cell lymphocyte proliferation, and the levels of interferon-γ and interleukin-4 cytokines. The results showed that live rMVA-mep elicited strongly immune responses in dose-dependent manner, and the highest level of immune responses was observed from the groups immunized with 107 TCID50 rMVA-mep among the experimental three concentrations. There were almost no difference of cytokines and neutralizing antibody titers among 107 TCID50 rMVA-mep, recombinant ED3 and inactivated JEV vaccine. It was noteworthy that rMVA-mep vaccination potentiates the Th1 and Th2-type immune responses in dose-dependent manner, and was sufficient to protect the mice survival against lethal JEV challenge. These findings demonstrated that rMVA-mep can produce adequate humoral and cellular immune responses, and protection in mice, which suggested that rMVA-mep might be an attractive candidate vaccine for preventing JEV infection.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 7%
United States 1 7%
Unknown 13 87%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 47%
Other 2 13%
Student > Postgraduate 1 7%
Student > Master 1 7%
Student > Bachelor 1 7%
Other 3 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 53%
Unspecified 2 13%
Biochemistry, Genetics and Molecular Biology 2 13%
Medicine and Dentistry 2 13%
Immunology and Microbiology 1 7%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 September 2012.
All research outputs
#11,130,768
of 12,515,237 outputs
Outputs from Virology Journal
#1,925
of 2,099 outputs
Outputs of similar age
#108,157
of 126,975 outputs
Outputs of similar age from Virology Journal
#28
of 36 outputs
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