Title |
High frequency of pre-existing neutralizing antibody responses in patients with dengue during an outbreak in Central Brazil
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Published in |
BMC Infectious Diseases, October 2016
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DOI | 10.1186/s12879-016-1867-6 |
Pubmed ID | |
Authors |
Angela Ferreira Lopes de Teive e Argolo, Valéria Christina de Rezende Féres, Marli Tenório Cordeiro, Lucimeire Antonelli da Silveira, Adriana Oliveira Guilarde, Ernesto Torres de Azevedo Marques, Wayner Vieira de Souza, Celina Maria Turchi Martelli |
Abstract |
This study aims to identify dengue neutralizing antibody response in patients with dengue from a well-characterized cohort during an outbreak in central Brazil, 2012-2013. We analyzed paired samples from 40 patients with severe dengue and 20 patients with dengue. Eligibility criteria were: IgM, NS1Ag and/or RT-PCR positivity and positive IgG result. Plaque reduction neutralization test (PRNT50) from DENV-1 to DENV-4 was performed to identify serotype-specific NAbs response. An infecting serotype was defined as ≥4-fold increase in DENV NAbs in paired samples. Monotypic response was classified as PRNT50 ≥ 1/20 to only one DENV serotype, and multitypic response was considered to be PRNT50 ≥ 1/20 to two or more serotypes simultaneously. Patients were mainly adults. Virological dengue infection was confirmed by RT-PCR: DENV-4(n = 14) and DENV-1(n = 10). Forty-four out of 60(73.3 %) patients had NAbs to DENV-4, DENV-1(68.3 %), DENV-2(68.3 %) and DENV-3(61.6 %) respectively. Fifteen percent of the cases presented monotypic response, whereas 85 % had multitypic response. DENV-4 infected-patients presented the greatest difference in PRNT50 titers compared with other serotypes. Pre-existing DENV NAbs was not correlated with disease severity. This was the first time that DENV-4 was implicated in an epidemic in the region. Our data indicates high exposure of multiple DENV serotypes in all age groups in the pre-dengue vaccine era and also previous to Zika virus introduction in Brazil. |
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Researcher | 15 | 22% |
Other | 6 | 9% |
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Student > Ph. D. Student | 4 | 6% |
Other | 10 | 14% |
Unknown | 8 | 12% |
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Pharmacology, Toxicology and Pharmaceutical Science | 5 | 7% |
Other | 10 | 14% |
Unknown | 11 | 16% |