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Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species.

Overview of attention for article published in Plant Methods, June 2014
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

twitter
10 tweeters

Citations

dimensions_citation
84 Dimensions

Readers on

mendeley
455 Mendeley
citeulike
1 CiteULike
Title
Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species.
Published in
Plant Methods, June 2014
DOI 10.1186/1746-4811-10-21
Pubmed ID
Authors

Adam Healey, Agnelo Furtado, Tal Cooper, Robert J Henry

Abstract

Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within their leaf tissue making genomic DNA extraction difficult. While many DNA extraction methods exist that contend with the presence of phenolics and polysaccharides, these methods rely on long incubations, multiple precipitations or commercially available kits to produce high molecular weight and contaminant-free DNA. In this protocol, we describe simple modifications to the established CTAB- based extraction method that allows for reliable isolation of high molecular weight genomic DNA from difficult to isolate plant species Corymbia (a eucalypt) and Coffea (coffee). The simplified protocol does not require multiple clean up steps or commercial based kits, and the isolated DNA passed stringent quality control standards for whole genome sequencing on Illumina HiSeq and TruSeq sequencing platforms.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 455 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 1%
Japan 2 <1%
Argentina 2 <1%
United Kingdom 2 <1%
Malaysia 2 <1%
Brazil 2 <1%
Netherlands 1 <1%
Norway 1 <1%
China 1 <1%
Other 9 2%
Unknown 428 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 104 23%
Researcher 81 18%
Student > Master 78 17%
Student > Bachelor 73 16%
Unspecified 40 9%
Other 79 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 263 58%
Biochemistry, Genetics and Molecular Biology 87 19%
Unspecified 49 11%
Immunology and Microbiology 15 3%
Environmental Science 13 3%
Other 28 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2017.
All research outputs
#1,700,487
of 8,982,225 outputs
Outputs from Plant Methods
#81
of 342 outputs
Outputs of similar age
#34,229
of 178,945 outputs
Outputs of similar age from Plant Methods
#3
of 15 outputs
Altmetric has tracked 8,982,225 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 342 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 178,945 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.