| Title |
Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
|
|---|---|
| Published in |
Plant Methods, January 2014
|
| DOI | 10.1186/1746-4811-10-21 |
| Pubmed ID | |
| Authors |
Adam Healey, Agnelo Furtado, Tal Cooper, Robert J Henry |
| Abstract |
Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within their leaf tissue making genomic DNA extraction difficult. While many DNA extraction methods exist that contend with the presence of phenolics and polysaccharides, these methods rely on long incubations, multiple precipitations or commercially available kits to produce high molecular weight and contaminant-free DNA. In this protocol, we describe simple modifications to the established CTAB- based extraction method that allows for reliable isolation of high molecular weight genomic DNA from difficult to isolate plant species Corymbia (a eucalypt) and Coffea (coffee). The simplified protocol does not require multiple clean up steps or commercial based kits, and the isolated DNA passed stringent quality control standards for whole genome sequencing on Illumina HiSeq and TruSeq sequencing platforms. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 27% |
| Germany | 1 | 9% |
| Brazil | 1 | 9% |
| Mexico | 1 | 9% |
| Unknown | 5 | 45% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 64% |
| Scientists | 3 | 27% |
| Science communicators (journalists, bloggers, editors) | 1 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 885 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | <1% |
| Malaysia | 2 | <1% |
| Japan | 2 | <1% |
| United Kingdom | 2 | <1% |
| Argentina | 2 | <1% |
| Norway | 1 | <1% |
| Bolivia, Plurinational State of | 1 | <1% |
| Italy | 1 | <1% |
| Brazil | 1 | <1% |
| Other | 9 | 1% |
| Unknown | 859 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 156 | 18% |
| Student > Bachelor | 141 | 16% |
| Researcher | 129 | 15% |
| Student > Master | 127 | 14% |
| Student > Doctoral Student | 37 | 4% |
| Other | 108 | 12% |
| Unknown | 187 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 370 | 42% |
| Biochemistry, Genetics and Molecular Biology | 176 | 20% |
| Unspecified | 23 | 3% |
| Environmental Science | 22 | 2% |
| Immunology and Microbiology | 21 | 2% |
| Other | 67 | 8% |
| Unknown | 206 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 December 2021.
All research outputs
#5,406,562
of 22,757,541 outputs
Outputs from Plant Methods
#293
of 1,080 outputs
Outputs of similar age
#62,409
of 305,255 outputs
Outputs of similar age from Plant Methods
#2
of 12 outputs
Altmetric has tracked 22,757,541 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,080 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,255 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.