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A Chinese herbal decoction, Jian-Pi-Yi-Shen, regulates the expressions of erythropoietin and pro-inflammatory cytokines in cultured cells

Overview of attention for article published in BMC Complementary and Alternative Medicine, April 2018
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Title
A Chinese herbal decoction, Jian-Pi-Yi-Shen, regulates the expressions of erythropoietin and pro-inflammatory cytokines in cultured cells
Published in
BMC Complementary and Alternative Medicine, April 2018
DOI 10.1186/s12906-018-2146-4
Pubmed ID
Authors

Jianping Chen, Amy G. W. Gong, Xiaoyan Liu, Zhonggui Li, Airong Qi, Tina T. X. Dong, Tiegang Yi, Karl W. K. Tsim, Shunmin Li

Abstract

A Chinese herbal formula, namely Jian-Pi-Yi-Shen (JPYS), has been clinically prescribed for patients with chronic kidney disease associated-anemia, and which can improve the patient's immunological system. However, the mechanisms of JPYS involved in anemia and immune response have not been investigated. To study the role of JPYS in regulating hematopoietic and immunological functions, we investigated its activities on the expressions of erythropoietin and pro-inflammatory cytokines in cultured cells. The standardized herbal extracts of JPYS (0-30 μg/ml) were applied onto cultured cells for 24-48 h. Total RNA was collected from the treated cells and subjected to real-time quantitative PCR analysis. Cultured HEK293T cells, transfected with a construct composed of hypoxia response element tagged with a luciferase gene, i.e. pHRE-Luc, were treated with JPYS extracts (1-30 μg/ml) for 24 h. The cell lysates were subjected to luciferase assay. The treatment with JPYS extract onto cultured HEK293T cells induced erythropoietin expression in a dose-dependent manner, having the highest response by ~ 50% of increase. In parallel, application of JPYS extract for 24 h stimulated expressions of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in cultured RAW 264.7 macrophages. In contrast, the pretreatment with JPYS extract suppressed expressions of IL-1β, IL-6, and TNF-α in lipopolysaccharide-induced macrophages. These results confirmed the hematopoietic function of JPYS in regulating erythropoietin expression, as well as the bidirectional immune-modulatory roles of JPYS by regulating the expression of pro-inflammatory cytokines in cultures.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Lecturer 1 20%
Student > Bachelor 1 20%
Other 1 20%
Student > Ph. D. Student 1 20%
Researcher 1 20%
Other 0 0%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 1 20%
Environmental Science 1 20%
Unspecified 1 20%
Decision Sciences 1 20%
Medicine and Dentistry 1 20%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 April 2018.
All research outputs
#11,997,013
of 13,534,507 outputs
Outputs from BMC Complementary and Alternative Medicine
#2,233
of 2,757 outputs
Outputs of similar age
#236,377
of 271,362 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#1
of 1 outputs
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