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Fluorescent nanodiamonds as a relevant tag for the assessment of alum adjuvant particle biodisposition

Overview of attention for article published in BMC Medicine, June 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (94th percentile)
  • Good Attention Score compared to outputs of the same age and source (70th percentile)

Mentioned by

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1 blog
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28 X users
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4 Facebook pages
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1 Google+ user
reddit
2 Redditors

Citations

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54 Dimensions

Readers on

mendeley
52 Mendeley
Title
Fluorescent nanodiamonds as a relevant tag for the assessment of alum adjuvant particle biodisposition
Published in
BMC Medicine, June 2015
DOI 10.1186/s12916-015-0388-2
Pubmed ID
Authors

Housam Eidi, Marie-Odile David, Guillemette Crépeaux, Laetitia Henry, Vandana Joshi, Marie-Hélène Berger, Mohamed Sennour, Josette Cadusseau, Romain K. Gherardi, Patrick A. Curmi

Abstract

Aluminum oxyhydroxide (alum) is a crystalline compound widely used as an immunologic adjuvant of vaccines. Concerns linked to alum particles have emerged following recognition of their causative role in the so-called macrophagic myofasciitis (MMF) lesion in patients with myalgic encephalomyelitis, revealing an unexpectedly long-lasting biopersistence of alum within immune cells and a fundamental misconception of its biodisposition. Evidence that aluminum-coated particles phagocytozed in the injected muscle and its draining lymph nodes can disseminate within phagocytes throughout the body and slowly accumulate in the brain further suggested that alum safety should be evaluated in the long term. However, lack of specific staining makes difficult the assessment of low quantities of bona fide alum adjuvant particles in tissues. We explored the feasibility of using fluorescent functionalized nanodiamonds (mfNDs) as a permanent label of alum (Alhydrogel(®)). mfNDs have a specific and perfectly photostable fluorescence based on the presence within the diamond lattice of nitrogen-vacancy centers (NV centers). As the NV center does not bleach, it allows the microspectrometric detection of mfNDs at very low levels and in the long-term. We thus developed fluorescent nanodiamonds functionalized by hyperbranched polyglycerol (mfNDs) allowing good coupling and stability of alum:mfNDs (AluDia) complexes. Specificities of AluDia complexes were comparable to the whole reference vaccine (anti-hepatitis B vaccine) in terms of particle size and zeta potential. In vivo, AluDia injection was followed by prompt phagocytosis and AluDia particles remained easily detectable by the specific signal of the fND particles in the injected muscle, draining lymph nodes, spleen, liver and brain. In vitro, mfNDs had low toxicity on THP-1 cells and AluDia showed cell toxicity similar to alum alone. Expectedly, AluDia elicited autophagy, and allowed highly specific detection of small amounts of alum in autophagosomes. The fluorescent nanodiamond technology is able to overcome the limitations of previously used organic fluorophores, thus appearing as a choice methodology for studying distribution, persistence and long-term neurotoxicity of alum adjuvants and beyond of other types of nanoparticles.

Timeline
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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
France 1 2%
Unknown 50 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 17%
Researcher 8 15%
Student > Master 7 13%
Student > Doctoral Student 5 10%
Other 3 6%
Other 7 13%
Unknown 13 25%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 6 12%
Agricultural and Biological Sciences 5 10%
Medicine and Dentistry 5 10%
Nursing and Health Professions 3 6%
Immunology and Microbiology 3 6%
Other 16 31%
Unknown 14 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 29. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2024.
All research outputs
#1,437,824
of 26,597,648 outputs
Outputs from BMC Medicine
#993
of 4,258 outputs
Outputs of similar age
#16,601
of 278,443 outputs
Outputs of similar age from BMC Medicine
#20
of 68 outputs
Altmetric has tracked 26,597,648 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,258 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 46.5. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,443 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 68 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.