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Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus

Overview of attention for article published in BMC Complementary and Alternative Medicine, June 2018
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Title
Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus
Published in
BMC Complementary and Alternative Medicine, June 2018
DOI 10.1186/s12906-018-2246-1
Pubmed ID
Authors

Parvaneh Mehrbod, Muna Ali Abdalla, Fatemeh Fotouhi, Masoumeh Heidarzadeh, Abimbola O. Aro, Jacobus N. Eloff, Lyndy J. McGaw, Folorunso O. Fasina

Abstract

Influenza infection is a major public health threat. The role of influenza A virus-induced inflammatory response in severe cases of this disease is widely recognized. Drug resistance and side effects of chemical treatments have been observed, resulting in increased interest in alternative use of herbal medications for prophylaxis against this infection. The South African medicinal plant, Rapanea melanophloeos (RM) (L.) Mez of the family Myrsinaceae was selected owing to its traditional use for the treatment of several diseases such as respiratory ailments and also previous preliminary studies of anti-influenza activity of its methanolic extract. The aim of this study was to investigate the immunomodulatory properties of a glycoside flavone isolated from RM against influenza A virus. The non-cytotoxic concentration of the quercetin-3-O-α-L-rhamnopyranoside (Q3R) was determined by MTT assay and tested for activity against influenza A virus (IAV) in simultaneous, pre-penetration and post-penetration combination treatments over 1 h incubation on MDCK cells. The virus titer and viral load targeting NP and M2 viral genes were determined using HA and qPCR, respectively. TNF-α and IL-27 as pro- and anti-inflammatory cytokines were measured at RNA and protein levels by qPCR and ELISA, respectively. Quercetin-3-O-α-L-rhamnopyranoside at 150 μg/ml decreased the viral titer by 6 logs (p < 0.01) in the simultaneous procedure. The NP and M2 genes copy numbers as viral target genes, calculated based on the Ct values and standard formula, significantly decreased in simultaneous treatment (p < 0.01). The expression of cytokines was also considerably affected by the compound treatment. This is the first report of quercetin-3-O-α-L-rhamnopyranoside from RM and its immunomodulatory properties against influenza A virus. Further research will focus on detecting the specific mechanism of virus-host interactions.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 2 33%
Researcher 2 33%
Student > Ph. D. Student 1 17%
Lecturer > Senior Lecturer 1 17%
Readers by discipline Count As %
Unspecified 3 50%
Agricultural and Biological Sciences 1 17%
Immunology and Microbiology 1 17%
Medicine and Dentistry 1 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2018.
All research outputs
#11,631,111
of 13,093,005 outputs
Outputs from BMC Complementary and Alternative Medicine
#2,161
of 2,643 outputs
Outputs of similar age
#234,136
of 270,367 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#1
of 1 outputs
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So far Altmetric has tracked 2,643 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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