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The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells

Overview of attention for article published in BMC Complementary and Alternative Medicine, June 2018
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Title
The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
Published in
BMC Complementary and Alternative Medicine, June 2018
DOI 10.1186/s12906-018-2250-5
Pubmed ID
Authors

Rabiatul Adawiyah Razali, Nik Ahmad Hafiz Nik Ahmad Eid, Turkambigai Jayaraman, Muhammad Asyrafi Amir Hassan, Nabilah Qistina Azlan, Nur Farhana Ismail, Nur Qisya Afifah Veronica Sainik, Muhammad Dain Yazid, Yogeswaran Lokanathan, Aminuddin Bin Saim, Ruszymah Bt Hj Idrus

Abstract

One of the molecular mechanisms involved in upper airway-related diseases is epithelial-to-mesenchymal transition (EMT). Olea europaea (OE) has anti-inflammatory properties and thus, great potential to prevent EMT. This study aimed to investigate the effect of OE on EMT in primary nasal human respiratory epithelial cells (RECs). Respiratory epithelial cells were isolated and divided into four groups: control (untreated), treated with 0.05% OE (OE group), EMT induced with 5 ng/ml of transforming growth factor beta-1 (TGFβ1 group) and treated with 5 ng/ml TGFβ1 + 0.05% OE (TGFβ1 + OE group). The effects of OE treatment on growth kinetics, morphology and protein expression in RECs were evaluated. Immunocytochemistry analysis was performed to quantitate the total percentage of E-cadherin and vimentin expression from day 1 to day 3. There were no significant differences between untreated RECs and OE-treated RECs in terms of their morphology, growth kinetics and protein expression. Induction with TGFβ1 caused RECs to have an elongated spindle shape, a slower proliferation rate, a higher expression of vimentin and a lower expression of E-cadherin compared with the control. Cells in the TGFβ1 + OE group had similar epithelial shape to untreated group however it had no significant differences in their proliferation rate when compared to TGFβ1-induced RECs. Cells treated with TGFβ1 + OE showed significantly reduced expression of vimentin and increased expression of E-cadherin compared with the TGFβ1 group (P < 0.05). The ability of OE to inhibit EMT in RECs was shown by TGFb1-induced EMT REC morphology, growth kinetics and protein expression markers (E-cadherin and vimentin) upon treatment with OE and TGFβ1. Therefore, this study could provide insight into the therapeutic potential of OE to inhibit pathological tissue remodelling and persistent inflammation.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 44%
Student > Master 2 22%
Student > Bachelor 1 11%
Other 1 11%
Researcher 1 11%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 44%
Medicine and Dentistry 3 33%
Unspecified 1 11%
Materials Science 1 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2018.
All research outputs
#10,605,829
of 13,322,622 outputs
Outputs from BMC Complementary and Alternative Medicine
#1,846
of 2,698 outputs
Outputs of similar age
#200,444
of 268,322 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#1
of 1 outputs
Altmetric has tracked 13,322,622 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,698 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them