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Molecular mechanism of anti-cancer activity of phycocyanin in triple-negative breast cancer cells

Overview of attention for article published in BMC Cancer, October 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (75th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

twitter
6 tweeters
facebook
5 Facebook pages

Citations

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26 Dimensions

Readers on

mendeley
59 Mendeley
Title
Molecular mechanism of anti-cancer activity of phycocyanin in triple-negative breast cancer cells
Published in
BMC Cancer, October 2015
DOI 10.1186/s12885-015-1784-x
Pubmed ID
Authors

Mathangi Ravi, Shilpa Tentu, Ganga Baskar, Surabhi Rohan Prasad, Swetha Raghavan, Prajisha Jayaprakash, Jeyaraman Jeyakanthan, Suresh K Rayala, Ganesh Venkatraman

Abstract

Triple-negative breast cancers represent an important clinical challenge, as these cancers do not respond to conventional endocrine therapies or other available targeted agents. Phycocyanin (PC), a natural, water soluble and non-toxic molecule is shown to have potent anti-cancer property. In this study, we determined the efficacy of PC as an anti-neoplastic agent in vitro on a series of breast cancer cell lines. We studied effects of PC in inducing DNA damage and apoptosis through western blot and qPCR. Also, anti-metastatic and anti-angiogenic properties were studied by classic wound healing and vasculogenic mimicry assays. We found that triple negative MDA-MB-231 cells were most sensitive to PC (IC50 : 5.98 ± 0.95 μM) as compared to other cells. They also showed decreased cell proliferation and reduced colony formation ability upon treatment with PC. Profile of Cell cycle analysis showed that PC caused G1 arrest which could be attributed to decreased mRNA levels of Cyclin E and CDK-2 and increased p21 levels. Mechanistic studies revealed that PC induced apoptosis as evident by increase in percentage of annexin positive cells, increase in γ-H2AX levels, and by changing the Bcl-2/Bax ratio followed by release of cytochrome C and increased Caspase 9 levels. MDA MB 231 cells treated with PC resulted in decreased cell migration and increased cell adhesive property and also showed anti-angiogenic effects. We also observed that PC suppressed cyclooxygenase-2 (COX-2) expression and prostaglandin E(2) production. All these biological effects of phycocyanin on MDA MB 231 cells could be attributed to decreased MAPK signaling pathway. We also observed that PC is non-toxic to non-malignant cells, platelets and RBC's. Taken together, these findings demonstrate, for the first time, that PC may be a promising anti-neoplastic agent for treatment of triple negative breast cancers.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 1 2%
Unknown 58 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 25%
Student > Master 13 22%
Student > Bachelor 5 8%
Researcher 4 7%
Other 4 7%
Other 12 20%
Unknown 6 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 27%
Biochemistry, Genetics and Molecular Biology 16 27%
Medicine and Dentistry 5 8%
Chemical Engineering 2 3%
Chemistry 2 3%
Other 8 14%
Unknown 10 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 May 2017.
All research outputs
#2,201,527
of 9,792,785 outputs
Outputs from BMC Cancer
#611
of 4,098 outputs
Outputs of similar age
#61,885
of 249,711 outputs
Outputs of similar age from BMC Cancer
#35
of 289 outputs
Altmetric has tracked 9,792,785 research outputs across all sources so far. Compared to these this one has done well and is in the 77th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,098 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 249,711 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 289 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.