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Neuroprotective effects of Paeonia Lactiflora extract against cell death of dopaminergic SH-SY5Y cells is mediated by epigenetic modulation

Overview of attention for article published in BMC Complementary and Alternative Medicine, July 2016
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Title
Neuroprotective effects of Paeonia Lactiflora extract against cell death of dopaminergic SH-SY5Y cells is mediated by epigenetic modulation
Published in
BMC Complementary and Alternative Medicine, July 2016
DOI 10.1186/s12906-016-1205-y
Pubmed ID
Authors

Gyuhwi Lee, Jong Cheon Joo, Bo Yoon Choi, Anders M. Lindroth, Soo Jung Park, Yoon Jung Park

Abstract

The Paeonia lactiflora extract (PLE) has been reported to have neuroprotective effect against neurodegeneration that are induced by cellular stress such as oxidative stress. Its underlying mechanisms, however, remain unclear. In latest decades, emerging evidence has suggested that epigenetic mechanisms play a key role in gene regulation in response to the cellular stress. We investigated whether epigenetic modulation was involved in neuronal cell death by the neurotoxicant, 1-Methyl-4-phenylpyridinium (MPP(+)), and the neuroprotective effect of PLE. Differentiated SH-SY5Y, which is a well-established dopaminergic cell line model, was treated with 0 ~ 200 μg/ml PLE for 4 h prior to MPP(+) treatment. The effect of PLE on cell viability was determined by MTT assays. Gene expression levels of oxidative stress responsive genes, such as Heme oxygenase 1 (HMOX1), and histone modifiers, such as histone acetyltransferases (HATs) and deacetylases (HDACs) were measured by quantitative RT PCR. In order to investigate the changes in epigenetic modifications, the acetylated lysine 9 (H3K9ac) and lysine 27 (H3K27ac) of Histone H3 were measured by western blot using histones extracted from the cells. MPP(+)-induced cell death in SH-SY5Y cells was significantly reduced by PLE pretreatment in a dose-dependent manner, indicating the potent neuroprotective effects of PLE. It was accompanied by induced expression of HMOX1. MPP(+) treatment increased the expression of HATs and consistently increased H3K9ac and H3K27ac of Histone H3. PLE pretreatment impeded the changes in H3K9ac and H3K27ac, coincided with increased expression of HDAC5 without changes in HAT expression. The results suggested that MPP(+)-induced cell death in the dopaminergic SH-SY5Y cells was related with transcriptional induction of HATs and increased histone H3 acetylation and that PLE might prevent the cells from MPP(+)-induced cell death via tempering histone H3 acetylation.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 50%
Student > Master 2 25%
Student > Ph. D. Student 1 13%
Researcher 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 38%
Biochemistry, Genetics and Molecular Biology 2 25%
Neuroscience 2 25%
Medicine and Dentistry 1 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2016.
All research outputs
#5,846,715
of 8,076,187 outputs
Outputs from BMC Complementary and Alternative Medicine
#1,333
of 2,024 outputs
Outputs of similar age
#167,250
of 258,901 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#47
of 88 outputs
Altmetric has tracked 8,076,187 research outputs across all sources so far. This one is in the 24th percentile – i.e., 24% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,024 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.7. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
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We're also able to compare this research output to 88 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.